货号 | EK1738 |
---|---|
产品名称 | Human Glypican-5/GPC5 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Glypican-5/GPC5 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, E25-T554 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | GPC5 |
基因全名 | glypican 5 |
Uniprot ID | P78333 |
指标别名 | Glypican-5; Secreted glypican-5; GPC5 |
背景资料 | Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1738-CAP | 96-well plate precoated with anti- human Glypican-5/GPC5 antibody | 1 |
EK1738-ST | Lyophilized recombinant human Glypican-5/GPC5 standard | 10ng/tube×2 |
EK1738-DA | Biotinylated anti- human Glypican-5/GPC5 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 60 | 226 | 938 | 62 | 243 | 967 |
Standard deviation | 2.58 | 11.07 | 50.7 | 4.15 | 16.77 | 72.5 |
CV(%) | 4.3% | 4.9% | 5.4% | 6.7% | 6.9% | 7.5% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.073 | 0.141 | 0.231 | 0.332 | 0.562 | 0.854 | 1.451 | 2.071 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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