货号 | EK1796 |
---|---|
产品名称 | Human EphA1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human EphA1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, K26-E547 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | EPHA1 |
基因全名 | EPH receptor A1 |
Uniprot ID | P21709 |
指标别名 | Ephrin type-A receptor 1, hEpha1, EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH; EPHA1; EPH; EPHT; EPHT1 |
背景资料 | EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1796-CAP | 96-well plate precoated with anti- human EphA1 antibody | 1 |
EK1796-ST | Lyophilized recombinant human EphA1 standard | 10ng/tube×2 |
EK1796-DA | Biotinylated anti- human EphA1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 190 | 1195 | 4507 | 244 | 1203 | 4632 |
Standard deviation | 8.55 | 56.2 | 238.9 | 14.2 | 74.59 | 352.03 |
CV(%) | 4.5% | 4.7% | 5.3% | 5.8% | 6.2% | 7.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.069 | 0.169 | 0.202 | 0.291 | 0.525 | 0.868 | 1.529 | 2.168 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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