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| 货号 | EK1760 |
|---|---|
| 产品名称 | Human Endorepellin/HSPG2 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human Endorepellin/HSPG2 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, E3687-S4391 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <0.05ng/ml |
| 检测范围 | 0.624ng/ml-40ng/ml |
| 基因名称 | HSPG2 |
| 基因全名 | heparan sulfate proteoglycan 2 |
| Uniprot ID | P98160 |
| 指标别名 | Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; HSPG2 |
| 背景资料 | Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1760-CAP | 96-well plate precoated with anti- human human Endorepellin/HSPG2 antibody | 1 |
| EK1760-ST | Lyophilized recombinant human human Endorepellin/HSPG2 standard | 40ng/tube×2 |
| EK1760-DA | Biotinylated anti- human human Endorepellin/HSPG2antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1098 | 3228 | 15773 | 1118 | 4325 | 18932 |
| Standard deviation | 58.19 | 132.3 | 1056.8 | 60.4 | 311.4 | 1438.83 |
| CV(%) | 5.3% | 4.1% | 6.7% | 5.4% | 7.2% | 7.6% |
| Concentration(ng/ml) | 0 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 | 40 |
| O.D. | 0.016 | 0.195 | 0.463 | 0.737 | 0.829 | 1.631 | 1.893 | 2.245 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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