货号 | EK1928 |
---|---|
产品名称 | Human Endoglycan/PODXL2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Endoglycan/PODXL2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, G33-T500 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | PODXL2 |
基因全名 | podocalyxin like 2 |
Uniprot ID | Q9NZ53 |
指标别名 | Podocalyxin-like protein 2; Endoglycan; PODXL2; UNQ1861/PRO3742 |
背景资料 | Podocalyxin like 2 is a protein that in humans is encoded by the PODXL2 gene. It is mapped to 3q21.3. This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1928-CAP | 96-well plate precoated with anti- human Endoglycan/PODXL2 antibody | 1 |
EK1928-ST | Lyophilized recombinant human Endoglycan/PODXL2 standard | 10ng/tube×2 |
EK1928-DA | Biotinylated anti- human Endoglycan/PODXL2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 205 | 1739 | 5795 | 210 | 1893 | 5983 |
Standard deviation | 8.82 | 109.56 | 283.96 | 9.24 | 119.26 | 442.74 |
CV(%) | 4.3% | 6.3% | 4.9% | 4.4% | 6.3% | 7.4% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.079 | 0.137 | 0.191 | 0.328 | 0.567 | 0.973 | 1.612 | 2.313 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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