货号 | EK2080 |
---|---|
产品名称 | Human DNER PicoKine ELISA Kit |
产品描述 | For quantitative detection of human DNER in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, N35-Y640 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | DNER |
基因全名 | delta/notch like EGF repeat containing |
Uniprot ID | Q8NFT8 |
指标别名 | Delta and Notch-like epidermal growth factor-related receptor; DNER; BET; UNQ262; PRO299 |
背景资料 | Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 2q36.3. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2080-CAP | 96-well plate precoated with anti- human DNER antibody | 1 |
EK2080-ST | Lyophilized recombinant human DNER standard | 20ng/tube×2 |
EK2080-DA | Biotinylated anti- human DNER antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 344 | 1501 | 6765 | 452 | 1768 | 8541 |
Standard deviation | 15.14 | 70.55 | 324.72 | 28.02 | 114.92 | 589.33 |
CV(%) | 4.4% | 4.7% | 4.8% | 6.2% | 6.5% | 6.9% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.009 | 0.119 | 0.209 | 0.403 | 0.749 | 1.135 | 1.557 | 2.129 |
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