货号 | EK1633 |
---|---|
产品名称 | Human Dermatopontin/DPT PicoKine ELISA Kit |
产品描述 | For quantitative detection of human DPT in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,Q19-V201 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 93.7pg/ml-6000pg/ml |
基因名称 | DPT |
基因全名 | dermatopontin |
Uniprot ID | Q07507 |
指标别名 | Dermatopontin; Tyrosine-rich acidic matrix protein; TRAMP; DPT |
背景资料 | Dermatopontin is a protein that in humans is encoded by the DPT gene. It’s an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1633-CAP | 96-well plate precoated with anti- human DPT antibody | 1 |
EK1633-ST | Lyophilized recombinant human DPT standard | 10ng/tubex2 |
EK1633-DA | Biotinylated anti- human DPT antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 100 | 977 | 2868 | 91 | 933 | 3012 |
Standard deviation | 7.1 | 46.89 | 169.21 | 7.91 | 58.77 | 234.93 |
CV(%) | 7.1% | 4.8% | 5.9% | 8.7% | 6.3% | 7.8% |
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.002 | 0.058 | 0.137 | 0.266 | 0.491 | 1.137 | 1.785 | 2.262 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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