货号 | EK1881 |
---|---|
产品名称 | Human CTRP9 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CTRP9 in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, Q20-P333 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 46.9pg/ml-3000pg/ml |
基因名称 | C1QTNF9 |
基因全名 | C1q and TNF related 9 |
Uniprot ID | P0C862 |
指标别名 | Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380 |
背景资料 | The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1881-CAP | 96-well plate precoated with anti- human CTRP9 antibody | 1 |
EK1881-ST | Lyophilized recombinant human CTRP9 standard | 10ng/tube×2 |
EK1881-DA | Biotinylated anti- human CTRP9 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 65 | 280 | 1368 | 73 | 291 | 1425 |
Standard deviation | 3.19 | 14.28 | 79.34 | 5.18 | 22.12 | 111.15 |
CV(%) | 4.9% | 5.1% | 5.8% | 7.1% | 7.6% | 7.8% |
Concentration(pg/ml) | 0 | 46.9 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.007 | 0.026 | 0.069 | 0.162 | 0.354 | 0.799 | 1.518 | 2.021 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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