货号 | EK1956 |
---|---|
产品名称 | Human CREG1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CREG1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, R32-Q220 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CREG1 |
基因全名 | cellular repressor of E1A stimulated genes 1 |
Uniprot ID | O75629 |
指标别名 | Protein CREG1; Cellular repressor of E1A-stimulated genes 1; CREG1; CREG; UNQ727/PRO1409 |
背景资料 | This gene is mapped to 1q24.2. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1956-CAP | 96-well plate precoated with anti- human CREG1 antibody | 1 |
EK1956-ST | Lyophilized recombinant human CREG1 standard | 20ng/tube×2 |
EK1956-DA | Biotinylated anti- human CREG1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 441 | 1500 | 8633 | 514 | 2089 | 9813 |
Standard deviation | 21.61 | 79.50 | 578.41 | 32.90 | 100.27 | 578.97 |
CV(%) | 4.9% | 5.3% | 6.7% | 6.4% | 4.8% | 5.9% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.054 | 0.248 | 0.416 | 0.674 | 0.928 | 1.438 | 1.953 | 2.224 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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