货号 | EK1887 |
---|---|
产品名称 | Human CPE PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CPE in For quantitative detection of human CPE in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L43-F476 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CPE |
基因全名 | carboxypeptidase E |
Uniprot ID | P16870 |
指标别名 | Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase |
背景资料 | This gene encodes a member of the M14 family of metallocarboxypeptidases. It is mapped to 4q32.3. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1887-CAP | 96-well plate precoated with anti- human CPE antibody | 1 |
EK1887-ST | Lyophilized recombinant human CPE standard | 10ng/tube×2 |
EK1887-DA | Biotinylated anti- human CPE antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 55 | 259 | 792 | 60 | 261 | 899 |
Standard deviation | 4.02 | 14.50 | 34.85 | 2.76 | 14.88 | 61.13 |
CV(%) | 7.3% | 5.6% | 4.4% | 4.6% | 5.7% | 6.8% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.013 | 0.068 | 0.119 | 0.202 | 0.409 | 0.787 | 1.459 | 2.189 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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