货号 | EK1861 |
---|---|
产品名称 | Human CPA2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CPA2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, S115-Y419 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | CPA2 |
基因全名 | carboxypeptidase A2 |
Uniprot ID | P48052 |
指标别名 | Carboxypeptidase A2; CPA2; |
背景资料 | Carboxypeptidase A2 is an enzyme that in humans is encoded by the CPA2 gene. This gene is mapped to 7q32.2. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1861-CAP | 96-well plate precoated with anti- human CPA2 antibody | 1 |
EK1861-ST | Lyophilized recombinant human CPA2 standard | 10ng/tube×2 |
EK1861-DA | Biotinylated anti- human CPA2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 108 | 747 | 2199 | 162 | 749 | 2549 |
Standard deviation | 4.75 | 34.36 | 112.15 | 10.69 | 53.93 | 188.63 |
CV(%) | 4.4% | 4.6% | 5.1% | 6.6% | 7.2% | 7.4% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.006 | 0.072 | 0.119 | 0.211 | 0.432 | 0.793 | 1.364 | 2.291 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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