货号 | EK1770 |
---|---|
产品名称 | Human CPA1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CPA1 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | E.coli, A111-Y419 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CPA1 |
基因全名 | carboxypeptidase A1 |
Uniprot ID | P15085 |
指标别名 | Carboxypeptidase A1 (EC:3.4.17.1); CPA1; CPA |
背景资料 | Carboxypeptidase A1 is an enzyme that in humans is encoded by the CPA1 gene. It is mapped to 7q32.2. This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1770-CAP | 96-well plate precoated with anti- human CPA1 antibody | 1 |
EK1770-ST | Lyophilized recombinant human CPA1 standard | 10ng/tube×2 |
EK1770-DA | Biotinylated anti- human CPA1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 280 | 862 | 3211 | 301 | 969 | 3874 |
Standard deviation | 11.76 | 37.07 | 131.65 | 19.87 | 65.89 | 294.42 |
CV(%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.011 | 0.061 | 0.119 | 0.207 | 0.401 | 0.742 | 1.481 | 2.259 |
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