货号 | EK1771 |
---|---|
产品名称 | Human Contactin-1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Contactin-1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, E21-S993 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CNTN1 |
基因全名 | contactin 1 |
Uniprot ID | Q12860 |
指标别名 | Contactin-1; Glycoprotein gp135; Neural cell surface protein F3; CNTN1 |
背景资料 | Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. This gene is mapped to 12q12. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1771-CAP | 96-well plate precoated with anti- human Contactin-1 antibody | 1 |
EK1771-ST | Lyophilized recombinant human Contactin-1 standard | 10ng/tube×2 |
EK1771-DA | Biotinylated anti- human Contactin-1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 304 | 1040 | 4977 | 310 | 1115 | 4983 |
Standard deviation | 19.15 | 59.28 | 238.9 | 20.46 | 81.4 | 383.7 |
CV(%) | 6.3% | 5.7% | 4.8% | 6.6% | 7.3% | 7.7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.007 | 0.225 | 0.336 | 0.696 | 1.119 | 1.911 | 2.220 | 2.395 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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