货号 | EK1957 |
---|---|
产品名称 | Human CNDP1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CNDP1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, S27-H507 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CNDP1 |
基因全名 | carnosine dipeptidase 1 |
Uniprot ID | Q96KN2 |
指标别名 | Beta-Ala-His dipeptidase; CNDP dipeptidase 1; Carnosine dipeptidase 1; Glutamate carboxypeptidase-like protein 2; Serum carnosinase; CNDP1; CN1; CPGL2; UNQ1915/PRO4380 |
背景资料 | Beta-Ala-His dipeptidase is an enzyme that in humans is encoded by the CNDP1 gene. It is mapped to 18q22.3. This gene encodes a member of the M20 metalloprotease family. The encoded protein is specifically expressed in the brain, is a homodimeric dipeptidase which was identified as human carnosinase. This gene contains trinucleotide (CTG) repeat length polymorphism in the coding region. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1957-CAP | 96-well plate precoated with anti- human CNDP1 antibody | 1 |
EK1957-ST | Lyophilized recombinant human CNDP1 standard | 20ng/tube×2 |
EK1957-DA | Biotinylated anti- human CNDP1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 613 | 2702 | 8855 | 620 | 2918 | 9790 |
Standard deviation | 30.04 | 143.21 | 602.14 | 44.02 | 131.31 | 528.66 |
CV(%) | 4.9% | 5.3% | 6.8% | 7.1% | 4.5% | 5.4% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.119 | 0.163 | 0.204 | 0.274 | 0.460 | 0.748 | 1.260 | 2.188 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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