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| 货号 | EK2051 |
|---|---|
| 产品名称 | Human CLEC14A PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human CLEC14A in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, E22-A490 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | CLEC14A |
| 基因全名 | C-type lectin domain containing 14A |
| Uniprot ID | Q86T13 |
| 指标别名 | C-type lectin domain family 14 member A; Epidermal growth factor receptor 5; EGFR-5; CLEC14A; C14orf27; EGFR5; UNQ236; PRO269 |
| 背景资料 | CLEC14A (C-type lectin domain family 14 member A; also EGFR5) is a 51 kDa (predicted) member of the C-type lectin domain family of proteins. This gene is mapped to 14q21.1. This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This family member plays a role in cell-cell adhesion and angiogenesis. It functions in filopodia formation, cell migration and tube formation. Due to its presence at higher levels in tumor endothelium than in normal tissue endothelium, it is considered to be a candidate for tumor vascular targeting. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2051-CAP | 96-well plate precoated with anti- human CLEC14A antibody | 1 |
| EK2051-ST | Lyophilized recombinant human CLEC14A standard | 10ng/tube×2 |
| EK2051-DA | Biotinylated anti- human CLEC14A antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 247 | 1236 | 4806 | 295 | 1245 | 4921 |
| Standard deviation | 11.12 | 70.45 | 221.08 | 20.95 | 85.91 | 314.94 |
| CV(%) | 4.5% | 5.7% | 4.6% | 7.1% | 6.9% | 6.4% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.033 | 0.157 | 0.245 | 0.475 | 0.844 | 1.293 | 1.735 | 2.365 |
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