货号 | EK1943 |
---|---|
产品名称 | Human CLEC11A PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CLEC11A in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A22-F323 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CLEC11A |
基因全名 | C-type lectin domain containing 11A |
Uniprot ID | Q9Y240 |
指标别名 | C-type lectin domain family 11 member A; C-type lectin superfamily member 3; Lymphocyte secreted C-type lectin; Osteolectin; Stem cell growth factor; p47; CLEC11A; CLECSF3; LSLCL; SCGF |
背景资料 | This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. It is mapped to 19q13.33. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1943-CAP | 96-well plate precoated with anti- human CLEC11A antibody | 1 |
EK1943-ST | Lyophilized recombinant human CLEC11A standard | 10ng/tube×2 |
EK1943-DA | Biotinylated anti- human CLEC11A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 270 | 1292 | 4732 | 284 | 1347 | 4986 |
Standard deviation | 19.44 | 81.40 | 212.94 | 15.05 | 86.21 | 209.41 |
CV(%) | 7.2% | 6.3% | 4.5% | 5.3% | 6.4% | 4.2% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.035 | 0.136 | 0.244 | 0.399 | 0.660 | 1.129 | 1.524 | 2.026 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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