货号 | EK1751 |
---|---|
产品名称 | Human ckmm PicoKine ELISA Kit |
产品描述 | For quantitative detection of human ckmm in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | 0 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.05mU/ml |
检测范围 | 0.15mU/ml-10mU/ml |
基因名称 | CKM |
基因全名 | creatine kinase, M-type |
Uniprot ID | P06732 |
指标别名 | Creatine kinase M-type; Creatine kinase M chain; Creatine kinase M chain; CPK-M; CKM; CKMM |
背景资料 | Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1751-CAP | 96-well plate precoated with anti- human ckmm antibody | 1 |
EK1751-ST | Lyophilized recombinant human ckmm standard | 10mU/tube×2 |
EK1751-DA | Biotinylated anti- human ckmm antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 Mea |
n(ulU/ml) | 233 | 1369 | 3636 | 242 | 1421 | 4520 |
Standard deviation | 9.77 | 88.99 | 147.5 | 13.31 | 96.6 | 329.96 |
CV(%) | 4.2% | 6.5% | 4.8% | 5.5% | 6.8% | 7.3% |
Concentration(mU/ml) | 0 | 0.15 | 0.31 | 0.62 | 1.25 | 2.5 | 5 | 10 |
O.D. | 0.030 | 0.068 | 0.109 | 0.183 | 0.384 | 0.872 | 1.829 | 2.737 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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