货号 | EK1953 |
---|---|
产品名称 | Human CHRDL1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CHRDL1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, E22-C450 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | CHRDL1 |
基因全名 | chordin like 1 |
Uniprot ID | Q9BU40 |
指标别名 | Chordin-like protein 1; Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1 |
背景资料 | Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1953-CAP | 96-well plate precoated with anti- human CHRDL1 antibody | 1 |
EK1953-ST | Lyophilized recombinant human CHRDL1 standard | 10ng/tube×2 |
EK1953-DA | Biotinylated anti- human CHRDL1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 146 | 511 | 1796 | 201 | 618 | 2851 |
Standard deviation | 7.59 | 27.08 | 100.58 | 13.07 | 41.41 | 196.72 |
CV(%) | 5.2% | 5.3% | 5.6% | 6.5% | 6.7% | 6.9% |
Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.088 | 0.134 | 0.176 | 0.265 | 0.402 | 0.702 | 1.263 | 2.099 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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