货号 | EK1933 |
---|---|
产品名称 | Human CFHR5 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CFHR5 in cell culture supernates, serum and plasma (heparin, EDTA) and urine. |
免疫原 | NSO, E19-E569 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | CFHR5 |
基因全名 | complement factor H related 5 |
Uniprot ID | Q9BXR6 |
指标别名 | Complement factor H-related protein 5; FHR-5; CFHR5; CFHL5; FHR5 |
背景资料 | Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1933-CAP | 96-well plate precoated with anti- human CFHR5 antibody | 1 |
EK1933-ST | Lyophilized recombinant human CFHR5 standard | 50ng/tube×2 |
EK1933-DA | Biotinylated anti- human CFHR5 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1183 | 8370 | 19761 | 1211 | 8545 | 22028 |
Standard deviation | 57.97 | 393.39 | 810.20 | 67.82 | 435.80 | 1013.29 |
CV(%) | 4.9% | 4.7% | 4.1% | 5.6% | 5.1% | 4.6% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.024 | 0.121 | 0.163 | 0.289 | 0.622 | 0.906 | 1.573 | 2.181 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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