货号 | EK1814 |
---|---|
产品名称 | Human CES2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CES2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q27-L559 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CES2 |
基因全名 | carboxylesterase 2 |
Uniprot ID | O00748 |
指标别名 | Cocaine esterase; Carboxylesterase 2; CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2; CES2; ICE |
背景资料 | Carboxylesterase 2 is an enzyme that in humans is encoded by the CES2 gene. This gene is mapped to 16q22.1. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1814-CAP | 96-well plate precoated with anti- human CES2 antibody | 1 |
EK1814-ST | Lyophilized recombinant human CES2 standard | 20ng/tube×2 |
EK1814-DA | Biotinylated anti- human CES2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 496 | 1686 | 8521 | 537 | 1912 | 9483 |
Standard deviation | 20.83 | 82.6 | 434.57 | 30.1 | 122.37 | 673.3 |
CV(%) | 4.2% | 4.9% | 5.1% | 5.6% | 6.4% | 7.1% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.054 | 0.248 | 0.416 | 0.674 | 0.928 | 1.438 | 1.953 | 2.224 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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