货号 | EK1948 |
---|---|
产品名称 | Human CD55 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CD55 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D35-S353 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CD55 |
基因全名 | CD55 molecule (Cromer blood group) |
Uniprot ID | P08174 |
指标别名 | Complement decay-accelerating factor; CD55; CR; DAF |
背景资料 | Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1948-CAP | 96-well plate precoated with anti- human CD55 antibody | 1 |
EK1948-ST | Lyophilized recombinant human CD55 standard | 20ng/tube×2 |
EK1948-DA | Biotinylated anti- human CD55 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 541 | 2056 | 8927 | 588 | 2124 | 9804 |
Standard deviation | 23.26 | 133.64 | 419.57 | 36.46 | 104.08 | 696.08 |
CV(%) | 4.3% | 6.5% | 4.7% | 6.2% | 4.9% | 7.1% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.134 | 0.176 | 0.203 | 0.259 | 0.377 | 0.667 | 1.200 | 1.989 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894