货号 | EK1811 |
---|---|
产品名称 | Human CD164 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CD164 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D24-D162 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 93.8pg/ml-6000pg/ml |
基因名称 | CD164 |
基因全名 | CD164 molecule |
Uniprot ID | Q04900 |
指标别名 | Sialomucin core protein 24; MUC-24; Endolyn; Multi-glycosylated core protein 24; MGC-24; MGC-24v; CD164 |
背景资料 | Sialomucin core protein 24 also known as endolyn or CD164, is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1811-CAP | 96-well plate precoated with anti- human CD1641 antibody | 1 |
EK1811-ST | Lyophilized recombinant human CD164 standard | 10ng/tube×2 |
EK1811-DA | Biotinylated anti- human CD164 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 149 | 717 | 2964 | 162 | 741 | 2982 |
Standard deviation | 8.2 | 29.4 | 168.95 | 10.04 | 48.17 | 217.7 |
CV(%) | 5.5% | 4.1% | 5.7% | 6.2% | 6.5% | 7.3% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.000 | 0.082 | 0.173 | 0.294 | 0.522 | 0.976 | 1.585 | 2.118 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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