货号 | EK1929 |
---|---|
产品名称 | Human Cathepsin V PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Cathepsin V in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, V18-V334 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 78pg/ml-5000pg/ml |
基因名称 | CTSV |
基因全名 | cathepsin V |
Uniprot ID | O60911 |
指标别名 | Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305 |
背景资料 | Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1929-CAP | 96-well plate precoated with anti- human Cathepsin V antibody | 1 |
EK1929-ST | Lyophilized recombinant human Cathepsin V standard | 10ng/tube×2 |
EK1929-DA | Biotinylated anti- human Cathepsin V antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 100 | 714 | 2346 | 136 | 823 | 2464 |
Standard deviation | 5.30 | 44.27 | 105.57 | 6.39 | 52.67 | 133.06 |
CV(%) | 5.3% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.096 | 0.132 | 0.216 | 0.379 | 0.609 | 1.011 | 1.828 | 2.542 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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