货号 | EK2099 |
---|---|
产品名称 | Human Cadherin-15 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Cadherin-15 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, L61-A606 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CDH15 |
基因全名 | cadherin 15 |
Uniprot ID | P55291 |
指标别名 | Cadherin-15; Cadherin-14; Muscle cadherin; M-cadherin; CDH15; CDH14; CDH3 |
背景资料 | Cadherin-15 is a protein that in humans is encoded by the CDH15 gene. It is mapped to 16q24.3. This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2099-CAP | 96-well plate precoated with anti- human Cadherin-15 antibody | 1 |
EK2099-ST | Lyophilized recombinant human Cadherin-15 standard | 10ng/tube×2 |
EK2099-DA | Biotinylated anti- human Cadherin-15 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 255 | 1011 | 3863 | 264 | 1095 | 4861 |
Standard deviation | 10.46 | 43.47 | 177.70 | 13.46 | 64.61 | 325.69 |
CV(%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.006 | 0.074 | 0.170 | 0.311 | 0.638 | 1.084 | 1.772 | 2.293 |
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