货号 | EK1941 |
---|---|
产品名称 | Human CA6 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CA6 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Q18-N308 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | CA6 |
基因全名 | carbonic anhydrase 6 |
Uniprot ID | P23280 |
指标别名 | Carbonic anhydrase 6; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase |
背景资料 | This gene is mapped to 1p36.23. The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1941-CAP | 96-well plate precoated with anti- human CA6 antibody | 1 |
EK1941-ST | Lyophilized recombinant human CA6 standard | 10ng/tube×2 |
EK1941-DA | Biotinylated anti- human CA6 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 228 | 1372 | 5130 | 249 | 1437 | 5217 |
Standard deviation | 14.14 | 72.72 | 241.11 | 15.19 | 106.34 | 266.07 |
CV(%) | 6.2% | 5.3% | 4.7% | 6.1% | 7.4% | 5.1% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.068 | 0.134 | 0.189 | 0.318 | 0.575 | 0.961 | 1.456 | 2.109 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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