货号 | EK1939 |
---|---|
产品名称 | Human CA4 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CA4 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, A19-S284 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | CA4 |
基因全名 | carbonic anhydrase 4 |
Uniprot ID | P22748 |
指标别名 | Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV |
背景资料 | Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 17q23.1. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositolanchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1939-CAP | 96-well plate precoated with anti- human CA4 antibody | 1 |
EK1939-ST | Lyophilized recombinant human CA4 standard | 20ng/tube×2 |
EK1939-DA | Biotinylated anti- human CA4 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 553 | 2529 | 8599 | 614 | 2686 | 9228 |
Standard deviation | 42.03 | 123.92 | 481.54 | 41.75 | 139.67 | 544.45 |
CV(%) | 7.6% | 4.9% | 5.6% | 6.8% | 5.2% | 5.9% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.066 | 0.107 | 0.159 | 0.255 | 0.449 | 0.795 | 1.426 | 2.116 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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