货号 | EK1938 |
---|---|
产品名称 | Human CA3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human CA3 in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. |
免疫原 | E.coli, A2-K260 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | CA3 |
基因全名 | carbonic anhydrase 3 |
Uniprot ID | P07451 |
指标别名 | Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III; CA3 |
背景资料 | Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1938-CAP | 96-well plate precoated with anti- human CA3 antibody | 1 |
EK1938-ST | Lyophilized recombinant human CA3 standard | 50ng/tube×2 |
EK1938-DA | Biotinylated anti- human CA3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1301 | 6028 | 24574 | 1319 | 6108 | 24732 |
Standard deviation | 53.34 | 373.74 | 1105.83 | 61.99 | 390.91 | 1335.53 |
CV(%) | 4.1% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.045 | 0.111 | 0.169 | 0.280 | 0.471 | 0.805 | 1.403 | 2.142 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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