0
The Bosterbio OneStep Human CA125 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CA125 with a 96-well strip plate that is pre-coated with antibody specific for CA125. The detection antibody is a HRP conjugated antibody specific for CA125. The capture antibody is monoclonal antibody from mouse, and the detection antibody is monoclonal antibody from mouse. The kit is analytically validated with ready to use reagents. To measure Human CA125, add standards and samples to the wells, then add the HRP conjugated detection antibody. Wash away the unbounded protein and HRP conjugated detection antibody. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. Upon addition of the substrate, the density of the yellow product is linearly propotional to Human CA125 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CA125 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Boster's ELISA Resource Center at https://www.bosterbio.com/elisatechnical-resource-center.
| Product Name | Human CA-125 OneStep ELISA Kit |
|---|---|
| SKU/Catalog Number | EK7029 |
| Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CA125. 96wells/kit, with removable strips. |
| Cite This Product | Human CA-125 OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7029) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 3.8 U/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 15-300 U/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | Serum *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity | Volume | Buffers |
| Anti-Human CA125 Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Anti- CA125 monoclonal antibody, Polystyrene micro-well plate |
| Human CA125 Standards(S1~S4) | 4 | 0.5ml | CA125 (from natural protein) (15，41，120, 300) U/ml, 0.02M PBS, 20% new-born calf serum |
| HRP Conjugated anti-Human CA125 antibody | 1 | 6ml | HRP Conjugated anti-Human CA125 antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, from mouse monoclonal antibody |
| Controls | 1 | 0.5ml | CA125 (from natural protein), 0.02M PBS, 20% new-born calf serum |
| 20X Wash Buffer Concentrate | 1 | 15ml | Neutral buffer, contains 0.5% Tween-20 |
| Color Developing Reagent A | 1 | 7ml | Contains 11mmol/L H2O2 |
| Color Developing Reagent B | 1 | 7ml | Contains 2mmol/L TMB |
| Stop Solution | 1 | 7ml | 2mol/L dilute sulphuric acid |
| Plate Sealers | 2 | Piece |
1. Microplate Reader capable of reading absorbance at 450nm.
2. Automated plate washer (optional)
3. Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples.
4. Deionized or distilledwater.
5. 500ml graduated cylinders.
6. Test tubes for dilution.
You can check the tissue specificity below for information on selecting positive and negative control.
*if product is indicated to react with multiple species, protein info is based on the human gene.
Click the images to enlarge.
Human CA125 ELISA Kit standard curve "> Human CA125 ELISA Kit standard curve
1. For Research Use Only. Not for use in diagnostic procedures.
2. For Laboratory use.
3. Not for Internal or External Use in Humans or Animals.
4. There should be no eating or drinking within work area.
5. Always wear gloves and a protective lab coat.
6. No pipetting should be done by mouth. Handle all specimens and reagents as potentially infectious and biohazardous.
7. Do not add sodium azide to samples as preservative.
8. Do not use external controls containing sodium azide.
9. Use disposable pipette tips to avoid contaminating chromogenic substrate reagent. Discard reagent if it turns blue.
10. Do not pour chromogenic substrate back into container after use.
11. Do not freeze reagents.
12. Do not mix reagents from different kit lot numbers.
13. Keep reagents out of direct sunlight.
14. Handle stop reagent with care, since it is corrosive.
15. Bring all reagents to room temperature.
16. Viscous forensic samples should always be diluted in phosphate buffered saline or distilled water prior to pipetting.
17. Ensure the bag containing the micro-plate strips and desiccant is sealed well, if only a few strips are used.
Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. This kit is for use with serum samples without additives only.
1. Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
Bring all specimens and kit reagents to room temperature (20-25°C) and gently mix.
1. Secure the desired number of coated wells in the holder. Dispense 50ul of CA125 standards, specimens, and controls into the appropriate wells.
2. Dispense 100ul Enzyme Conjugate Reagent into each well.
3. Mix gently for 30 seconds. It is very important to have complete mixing in this setup.
4. Incubate for 60 minutes.
5. Remove the incubation mixture by emptying the plate content into a waste container.
6. Remove liquid from all wells. Wash wells three times with 300ul of 1X wash buffer. Blot on absorbance paper or paper towel.
7. Strike the microtiter plate sharply onto absorbent paper or paper towels to remove all residual liquid droplets.
8. Dispense 100ul of TMB Reagent into each well. Gently mix for 10 seconds. Incubate at room temperature, in the dark, for 15 minutes.
9. Stop the reaction by adding 50ul of Stop Solution to each well.
10. Read the absorbance at 450nm (using a reference wavelength of 630nm) with a microtiter plate absorbance reader within 15 minutes.
1. Calculate the average absorbance values (A450) for each set of reference standards, control, and samples.
2. Construct a standard curve by plotting the mean absorbance obtained from each reference standard standard against its concentration in U/ml on linear graph paper, with absorbance on the vertical (y) axis and concentration on the horizontal (x) axis.
3. Using the mean absorbance value for each sample, determine the corresponding concentration
2. To construct the standard curve, plot the absorbance for ACT standards (vertical
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
