货号 | EK1934 |
---|---|
产品名称 | Human C1S PicoKine ELISA Kit |
产品描述 | For quantitative detection of human C1S in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. |
免疫原 | NSO, E16-D688 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | C1S |
基因全名 | complement C1s |
Uniprot ID | P09871 |
指标别名 | Complement C1s subcomponent; C1 esterase; Complement component 1 subcomponent s; C1S |
背景资料 | Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1934-CAP | 96-well plate precoated with anti- human C1S antibody | 1 |
EK1934-ST | Lyophilized recombinant human C1S standard | 50ng/tube×2 |
EK1934-DA | Biotinylated anti- human C1S antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1236 | 4483 | 22854 | 1393 | 4567 | 22796 |
Standard deviation | 76.63 | 300.36 | 1028.43 | 82.19 | 324.26 | 980.23 |
CV(%) | 6.2% | 6.7% | 4.5% | 5.9% | 7.1% | 4.3% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.025 | 0.173 | 0.347 | 0.676 | 1.261 | 1.603 | 2.171 | 1.928 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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