货号 | EK1954 |
---|---|
产品名称 | Human C1R PicoKine ELISA Kit |
产品描述 | For quantitative detection of human C1R in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. |
免疫原 | NSO, S18-D705 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 1.56ng/ml-100ng/ml |
基因名称 | C1R |
基因全名 | complement C1r |
Uniprot ID | P00736 |
指标别名 | Complement C1r subcomponent; Complement component 1 subcomponent r; C1R |
背景资料 | Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. It is mapped to 12p13.31. In humans, C1r is encoded by the C1R gene. C1r along with C1q and C1s form the C1 complex, which is the first component of the serum complement system. C1r is an enzyme that activates C1s to its active form, by proteolytic cleavage.C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1954-CAP | 96-well plate precoated with anti- human C1R antibody | 1 |
EK1954-ST | Lyophilized recombinant human C1R standard | 100ng/tube×2 |
EK1954-DA | Biotinylated anti- human C1R antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2705 | 11224 | 42475 | 3058 | 11987 | 42819 |
Standard deviation | 129.84 | 583.65 | 1953.85 | 186.54 | 707.23 | 2740.42 |
CV(%) | 4.8% | 5.2% | 4.6% | 6.1% | 5.9% | 6.4% |
Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.051 | 0.096 | 0.127 | 0.207 | 0.363 | 0.646 | 1.115 | 1.716 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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