0
The Bosterbio OneStep ELISA kit is a solid phase direct ELISA sandwich kit. Instead of adding samples detection antibody and ABC-HRP separately, The OneStep ELISA kit allows the user to add standards, samples and controls to wells in one step, along with the incubation buffer. After a simple washing step, an enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.
| Product Name | Human Beta-2 Microglobulin OneStep ELISA Kit |
|---|---|
| SKU/Catalog Number | EK7023 |
| Description | Human Beta-2 Microglobulin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips. |
| Cite This Product | Human Beta-2 Microglobulin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7023) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 0.625 ?g/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 0.625-10 ?g/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | Serum and Plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity |
| Microwells coated Murine monoclonal anti-B2 MG antibody | 12x8x1 |
| B2MG Reference Standards: 0, 0.625, 1.25, 2.5, 5, and 10 | 1 ml |
| Sample Diluent, 100 ml. | 100 ml |
| Enzyme Conjugate Reagent, 22 ml | 22 ml |
| TMB Reagent (One-Step), 11 ml | 11 ml |
| Stop Solution (1N HCl), 11 ml. | 11 ml |
| Wash concentrate 20X: 1 bottle | 25 ml |
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. ELISA reader capable of reading absorbance at 450nm
5. Absorbance paper or paper towel
6. Graph paper
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | B2M |
|---|---|
| Uniprot ID | P61769 |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Click the images to enlarge.
1. Potential for Research Use Only. Not for use in diagnostic procedures.
2. For Laboratory use.
3. Not for Internal or External Use in Humans or Animals.
4. There should be no eating or drinking within work area.
5. Always wear gloves and a protective lab coat.
6. No pipetting should be done by mouth. Handle all specimens and reagents as potentially infectious and biohazardous.
7. Do not add sodium azide to samples as preservative.
8. Do not use external controls containing sodium azide.
9. Use disposable pipette tips to avoid contaminating chromogenic substrate reagent. Discard reagent if it turns blue.
10. Do not pour chromogenic substrate back into container after use.
11. Do not freeze reagents.
12. Do not mix reagents from different kit lot numbers.
13. Keep reagents out of direct sunlight.
14. Handle stop reagent with care, since it is corrosive.
15. Bring all reagents to room temperature.
16. Viscous forensic samples should always be diluted in phosphate buffered saline or distilled water prior to pipetting.
17. Ensure the bag containing the micro-plate strips and desiccant is sealed well, if only a few strips are used.
1. Blood should be drawn using standard venipuncture techniques and the serum should be separated from the red blood cells as soon as practical. Avoid grossly hemolytic, lipidic or turbid samples.
2. Typicaly, specimens should be capped and may be stored for up to 48 hour at 2-8°C prior to assaying. Specimens held for a longer time can be frozen at -20°C for up to 6 months prior to assay. Thawed samples should be inverted several times to mix prior to testing.
3. Collect urine samples and store at 2-8°C for up to 5 days or at -20°C for longer periods. Urine samples are calculate unknowns. Each laboratory must provide its own data and standard curve in each experiment.
Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
1. All reagents should be brought to room temperature (20-25°C) before use. All reagents should be mixed by gently inverting or swirling prior to use. Do not induce foaming.
2. Reconstitute each lyophilized standard with 1.0 ml-distilled water. Allow the reconstituted material to stand for at least 20 minutes and mix gently. Reconstituted standards will be stable for up to 30 days when stored sealed at 2-8°C.
1. Samples of patient serum, plasma and control serum need to be diluted before use for best results. Prepare a series of small tubes (such as 1.5 ml microcentrifuge tubes) and mix 10ul serum with 1.0 ml Sample Diluent (101 fold dilution). Do not dilute the standards, they have already been pre-diluted 101 fold.
2. Secure the desired number of coated wells in the holder.
3. Dispense 20ul of standards, diluted specimens, and diluted controls into appropriate wells.
4. Dispense 200ul of Sample Diluent into each well.
5. Thoroughly mix for 30 seconds. It is very important to mix them completely.
6. Incubate at 37°C for 30 minutes.
7. Remove the incubation mixture by flicking plate contents into a waste container.
8. Remove liquid from all wells. Wash wells three times with 300ul of 1X wash buffer. Blot on absorbance paper or paper towel.
9. Strike the wells sharply onto absorbent paper or paper towels to remove all residual liquid droplets.
10. Dispense 200ul of Enzyme Conjugate Reagent into each well. Gently mix for 10 seconds.
11. Incubate at 37°C for 30 minutes.
12. Remove the contents and wash the plate as described in step 7, 8, and 9.
13. Dispense 100ul TMB Reagent into each well.
14. Gently mix for 10 seconds.
15. Incubate at room temperature in the dark for 20 minutes.
16. Stop the reaction by adding 100ul of Stop Solution to each well.
17. Gently mix for 10 seconds. It is important to make sure that all the blue color changes to yellow color completely.
18. Read absorbance at 450nm with a microtiter well reader within 15 minutes.
1. Calculate the mean absorbance value (A450) for each set of reference standards, controls and patient samples. The standard curve is constructed as follows: against its concentration in .g/ml on graph paper, with absorbance values on the vertical or Y axis, and concentrations on the horizontal or X axis.
3. Use the mean absorbance values for each specimen to determine the corresponding concentration of
2. To construct the standard curve, plot the absorbance for fT4 standards (vertical axis)
1. Urine Samples need 10 fold Dilution with the Sample Diluent (i.e. 50ul urine + 450ul Sample Diluentl).
2. Construct a standard curve by plotting the mean absorbance obtained for each reference standard
1. Calculate the mean absorbance value (A450) for each reference standards, controls and patient samples. The standard curve is constructed as follows: against its concentration in .g/ml on graph paper, with absorbance values on the vertical or Y axis, and concentrations on the horizontal or X axis.
3. Use the mean absorbance values for each specimen to determine the corresponding concentration of .2MG in .g/ml. Divide the calculated values by 10.1 (Since the .-2 Microglobulin standards have been prediluted 101 fold, the results obtained from urine samples should be further divided by 10.1). represented. This curve should not be used in lieu of standard curve run with each assay. the real value will be 2.40 .g/ml . 10.1 = 0.238 .g/ml.3.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
