货号 | EK1691 |
---|---|
产品名称 | Human B2M PicoKine ELISA Kit |
产品描述 | For quantitative detection of human B2M in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and human milk. |
免疫原 | Plasma |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | B2M |
基因全名 | beta-2-microglobulin |
Uniprot ID | P61769 |
指标别名 | Beta-2-microglobulin; Beta-2-microglobulin form pI 5.3; B2M; CDABP0092; HDCMA22P |
背景资料 | Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells). In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1691-CAP | 96-well plate precoated with anti- Human B2M antibody | 1 |
EK1691-ST | Lyophilized recombinant Human B2M standard | 10ng/tubex2 |
EK1691-DA | Biotinylated anti- Human B2M antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 293 | 1070 | 5097 | 310 | 1103 | 4757 |
Standard deviation | 21.97 | 62.06 | 219.17 | 26.97 | 69.48 | 285.42 |
CV(%) | 7.5% | 5.8% | 4.3% | 8.7% | 6.3% | 6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.049 | 0.113 | 0.184 | 0.225 | 0.523 | 0.766 | 1.328 | 2.253 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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