货号 | EK2078 |
---|---|
产品名称 | Human ARSB PicoKine ELISA Kit |
产品描述 | For quantitative detection of human ARSB in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, S37-M533 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | ARSB |
基因全名 | arylsulfatase B |
Uniprot ID | P15848 |
指标别名 | Arylsulfatase B; ASB; N-acetylgalactosamine-4-sulfatase; G4S; ARSB |
背景资料 | Arylsulfatase B is an enzyme associated with mucopolysaccharidosis VI. It is mapped to 5q14.1. Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2078-CAP | 96-well plate precoated with anti- human ARSB antibody | 1 |
EK2078-ST | Lyophilized recombinant human ARSB standard | 10ng/tube×2 |
EK2078-DA | Biotinylated anti- human ARSB antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 18 | 68 | 470 | 25 | 84 | 481 |
Standard deviation | 0.95 | 3.81 | 27.26 | 1.65 | 5.80 | 35.11 |
CV(%) | 5.3% | 5.6% | 5.8% | 6.6% | 6.9% | 7.3% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.072 | 0.157 | 0.218 | 0.346 | 0.646 | 1.088 | 1.638 | 2.230 |
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