货号 | EK2077 |
---|---|
产品名称 | Human ARSA PicoKine ELISA Kit |
产品描述 | For quantitative detection of human ARSA in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, R19-A507 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | ARSA |
基因全名 | arylsulfatase A |
Uniprot ID | P15289 |
指标别名 | Arylsulfatase A; ASA; Cerebroside-sulfatase; ARSA |
背景资料 | Arylsulfatase A (or cerebroside-sulfatase) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate into cerebroside and sulfate. In humans, arylsulfatase A is encoded by the ARSA gene. It is mapped to 22q13.33. The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2077-CAP | 96-well plate precoated with anti- human ARSA antibody | 1 |
EK2077-ST | Lyophilized recombinant human ARSA standard | 20ng/tube×2 |
EK2077-DA | Biotinylated anti- human ARSA antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 575 | 1965 | 6227 | 581 | 2018 | 7737 |
Standard deviation | 25.30 | 90.39 | 292.67 | 32.54 | 117.04 | 487.43 |
CV(%) | 4.4% | 4.6% | 4.7% | 5.6% | 5.8% | 6.3% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.058 | 0.132 | 0.186 | 0.310 | 0.586 | 1.072 | 1.846 | 2.411 |
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