货号 | EK1745 |
---|---|
产品名称 | Human Annexin A1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Annexin A1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A2-N346 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <100pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | ANXA1 |
基因全名 | annexin A1 |
Uniprot ID | P04083 |
指标别名 | Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35; ANXA1; ANX1; LPC1 |
背景资料 | ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1745-CAP | 96-well plate precoated with anti- human Annexin A1 antibody | 1 |
EK1745-ST | Lyophilized recombinant human Annexin A1 standard | 10ng/tube×2 |
EK1745-DA | Biotinylated anti- human Annexin A1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 299 | 1474 | 5209 | 303 | 1499 | 5311 |
Standard deviation | 12.56 | 64.86 | 276.1 | 17.9 | 92.9 | 414.3 |
CV(%) | 4.2% | 4.4% | 5.3% | 5.9% | 6.2% | 7.8% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.066 | 0.196 | 0.245 | 0.452 | 0.861 | 1.437 | 1.943 | 2.275 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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