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Human ANGPTL7 PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2035

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

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Product Brief

  • 产品概况

    货号 EK2035
    产品名称 Human ANGPTL7 PicoKine ELISA Kit
    产品描述 For quantitative detection of human ANGPTL7 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 CHO, Q27-P346
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 78pg/ml-5000pg/ml
    基因名称 ANGPTL7
    基因全名 angiopoietin like 7
    Uniprot ID O43827
    指标别名 Angiopoietin-related protein 7; Angiopoietin-like factor; Angiopoietin-like protein 7; Cornea-derived transcript 6 protein; ANGPTL7; CDT6; UNQ313; PRO356
    背景资料 Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 1p36.22. Angiopoietin-like Protein 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted, 45‑50 kDa glycoprotein member of the angiopoietin‑like family of molecules. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain. ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera. Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha. ANGPTL7 expression is up‑regulated under conditions of increased ocular pressure.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2035-CAP 96-well plate precoated with anti- human ANGPTL7 antibody 1
    EK2035-ST Lyophilized recombinant human ANGPTL7 standard 10ng/tube×2
    EK2035-DA Biotinylated anti- human ANGPTL7 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 140 644 1907 145 639 2253
    Standard deviation 6.86 27.69 97.26 9.57 35.15 173.48
    CV(%) 4.9% 4.3% 5.1% 6.6% 5.5% 7.7%

    Human ANGPTL7 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 78 156 312 625 1250 2500 5000
    O.D. 0.029 0.059 0.101 0.192 0.389 0.793 1.508 2.276
    *典型数据仅供参考。用户需要自己建立标准曲线。

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