货号 | EK1993 |
---|---|
产品名称 | Human ANGPTL6 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human ANGPTL6 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | CHO, Ala20-Leu470 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | ANGPTL6 |
基因全名 | angiopoietin like 6 |
Uniprot ID | Q8NI99 |
指标别名 | Angiopoietin-related protein 6; Angiopoietin-like protein 6; Angiopoietin-related growth factor; Angiopoietin-related protein 5; ANGPTL6; AGF; ARP5; UNQ152; PRO178 |
背景资料 | Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain and a fibrinogen-like domain. It is mapped to 19p13.2. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum. Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure. Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. An integrin-binding RGD motif located within the fibrinogen-like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia. Serum ANGPTL6 levels are also elevated during obesity. ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells. ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1993-CAP | 96-well plate precoated with anti- human ANGPTL6 antibody | 1 |
EK1993-ST | Lyophilized recombinant human ANGPTL6 standard | 2ng/tube×2 |
EK1993-DA | Biotinylated anti- human ANGPTL6 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 46 | 215 | 874 | 49 | 221 | 896 |
Standard deviation | 2.44 | 10.54 | 35.83 | 3.23 | 12.38 | 49.28 |
CV(%) | 5.3% | 4.9% | 4.1% | 6.6% | 5.6% | 5.5% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.019 | 0.189 | 0.265 | 0.494 | 0.888 | 1.642 | 2.372 | 2.895 |
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