货号 | EK1514 |
---|---|
产品名称 | Human alpha-defensin/DEFA1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human DEFA1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,D39-C94 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | DEFA1 |
基因全名 | defensin alpha 1 |
Uniprot ID | P59665 |
指标别名 | Neutrophil defensin 1; Defensin, alpha 1; HNP-1; HP-1; HP1; HP 1-56; Neutrophil defensin 2; HNP-2; HP-2; HP2; DEFA1; DEF1; DEFA2; MRS; DEFA1B |
背景资料 | Defensin is a human protein that is encoded by the DEFA1 gene. Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1514-CAP | 96-well plate precoated with anti- human DEFA1 antibody | 1 |
EK1514-ST | Lyophilized recombinant human DEFA1 standard | 10ng/tubex2 |
EK1514-DA | Biotinylated anti- human DEFA1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 462 | 1682 | 9496 | 635 | 1897 | 9984 |
Standard deviation | 21.71 | 89.15 | 531.78 | 42.55 | 96.75 | 479.23 |
CV(%) | 4.7% | 5.3% | 5.6% | 6.7% | 5.1% | 4.8% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.091 | 0.139 | 0.172 | 0.203 | 0.353 | 0.633 | 1.277 | 2.163 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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