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| 货号 | EK1870 |
|---|---|
| 产品名称 | Human AKR1C4 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human AKR1C4 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, M1-Y323 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | AKR1C4 |
| 基因全名 | aldo-keto reductase family 1 member C4 |
| Uniprot ID | P17516 |
| 指标别名 | Aldo-keto reductase family 1 member C4; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4; CHDR |
| 背景资料 | 3α-Hydroxysteroid dehydrogenase (3α-HSD or aldo-keto reductase family 1 member C4) is an enzyme that in humans is encoded by the AKR1C4 gene. It is mapped to 10p15.1. This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1870-CAP | 96-well plate precoated with anti- human AKR1C4 antibody | 1 |
| EK1870-ST | Lyophilized recombinant human AKR1C4 standard | 10ng/tube×2 |
| EK1870-DA | Biotinylated anti- human AKR1C4 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 17 | 71 | 407 | 31 | 95 | 441 |
| Standard deviation | 0.75 | 3.27 | 19.13 | 1.74 | 5.51 | 27.78 |
| CV(%) | 4.4% | 4.6% | 4.7% | 5.6% | 5.8% | 6.3% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.028 | 0.111 | 0.184 | 0.328 | 0.630 | 1.072 | 1.754 | 2.417 |
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