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| 货号 | EK1465 |
|---|---|
| 产品名称 | Human AGT/Serpin A8 PicoKine ELISA Kit |
| 产品描述 | For Quantitative Detection of human AGT in cell culture supernates, serum and plasma (EDTA). |
| 免疫原 | NSO,D34-A485 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | AGT |
| 基因全名 | angiotensinogen |
| Uniprot ID | P01019 |
| 指标别名 | Angiotensinogen; Serpin A8; Angiotensin-1; Angiotensin 1-10; Angiotensin I; Ang I; Angiotensin-2; Angiotensin 1-8; Angiotensin II; Ang II; Angiotensin-3; Angiotensin 2-8; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II; Angiotensin-4; Angiotensin 3-8; Angiotensin IV; Ang IV; Angiotensin 1-9; Angiotensin 1-7; Angiotensin 1-5; Angiotensin 1-4; AGT; SERPINA8 |
| 背景资料 | AGT(ANGIOTENSINOGEN), also called SERPINA8, is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1465-CAP | 96-well plate precoated with anti- human AGT antibody | 1 |
| EK1465-ST | Lyophilized recombinant human AGT standard | 20ng/tube×2 |
| EK1465-DA | Biotinylated anti- human AGT antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 364 | 2379 | 7370 | 417 | 2441 | 8736 |
| Standard deviation | 18.93 | 126.09 | 405.35 | 25.44 | 156.22 | 585.31 |
| CV(%) | 5.2% | 5.3% | 5.5% | 6.1% | 6.4% | 6.7% |
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.081 | 0.159 | 0.281 | 0.412 | 0.764 | 1.116 | 1.776 | 2.485 |
典型操作程序：
1?准备试剂和标准品
2?每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3?每孔添加100ul生物素标记抗体，37℃反应60分钟。
4?0.01M TBS洗涤3次。
5?每孔添加100ulABC，37℃反应30分钟。
6?0.01M TBS洗涤5次。
7?TMB37℃避光反应30分钟以内。
8?加入TMB终止液，读数。
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