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| 货号 | A08965-1 |
|---|---|
| 产品名称 | Anti-IGLL1 Antibody |
| 基因名 | IGLL1 |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 分子量 | 23KD |
| 免疫原 | E. coli-derived human IGLL1 recombinant protein (L38-S213). |
| 内容 | 500ug/ml;Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500ug/ml |
| 产品形态 | Lyophilized |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Immunoglobulin lambda-like polypeptide 1 is a protein that in humans is encoded by the IGLL1 gene. It is mapped to 22q11.23. The preB cell receptor is found on the surface of proB and preB cells, where it is involved in transduction of signals for cellular proliferation, differentiation from the proB cell to the preB cell stage, allelic exclusion at the Ig heavy chain gene locus, and promotion of Ig light chain gene rearrangements. The preB cell receptor is composed of a membrane-bound Ig mu heavy chain in association with a heterodimeric surrogate light chain. This gene encodes one of the surrogate light chain subunits and is a member of the immunoglobulin gene superfamily. This gene does not undergo rearrangement. Mutations in this gene can result in B cell deficiency and agammaglobulinemia, an autosomal recessive disease in which few or no gamma globulins or antibodies are made. Two transcript variants encoding different isoforms have been found for this gene. |
| 研究类别 | 1. Kudo, A., Melchers, F. A second gene, Vpre-B in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes. EMBO J. 6: 2267-2272, 1987. 2. Linnebank, M., Tschiedel, E., Haberle, J., Linnebank, A., Willenbring, H., Kleijer, W. J., Koch, H. G. Argininosuccinate lyase (ASL) deficiency: mutation analysis in 27 patients and a completed structure of the human ASL gene. Hum. Genet. 111: 350-359, 2002.3. Mattei, M.-G., Fumoux, F., Roeckel, N., Fougereau, M., Schiff, C. The human pre-B-specific lambda-like cluster is located in the 22q11.2-22q12.3 region, distal to the IgC-lambda locus. Genomics 9: 544-546, 1991. |
| Uniprot ID | IGLL1: P15814 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1077) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry in paraffin section: | 1:50-400 |
| Direct ELISA： | 1:100-1000 |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
.)
Figure 1. Western blot analysis of IGLL1 using anti-IGLL1 antibody (A08965-1). Lane 1: human placenta tissue lysates. anti-IGLL1 antigen affinity purified polyclonal antibody (Catalog # A08965-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for IGLL1 at approximately 26KD. The expected band size for IGLL1 is at 23KD.
.)
Figure 2. IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).IGLL1 was detected in paraffin-embedded section of human B lymphocytic tumor tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
.)
Figure 3. IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).IGLL1 was detected in paraffin-embedded section of human tonsil tissue.Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
.)
Figure 4. IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).IGLL1 was detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
.)
Figure 5. IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).IGLL1 was detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
.)
Figure 6. IHC analysis of IGLL1 using anti-IGLL1 antibody (A08965-1).IGLL1 was detected in paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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