Sep-Pak Alumina B Plus Light cartridges contain a highly active grade of alumina with a basic surface chemistry. The aluminum oxide surface provides an extremely polar surface for analyte retention and has properties of a Lewis acid. This normal phase sorbent is similar in use to silica; however, it is slightly more stable than unfunctionalized silica under high pH conditions. This basic alumina is also low-capacity ion exchanger in aqueous media. Sep-Pak Alumina B Vac cartridges can be used to extract phospholipids, steroids, and catecholamines. Since Sep-Pak Alumina B is unaffected by high-energy radioactivity, these cartridges can be used for radioactive compound isolation. These cartridges have a distinctive finned outer body and a reduced internal diameter, which results in an interstitial volume about one-third that of the corresponding Plus-style cartridge. This design allows you to elute fractions in a minimal volume to improve recoveries and reduce solvent consumption, a benefit especially for applications where samples are limited or where excessive dilution is a concern.
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