Waters MassPREP On-Line Desalting Cartridges can effectively desalt proteins prior to LC/MS analyses. Because non-volatile salts (e.g., NaCl) can suppress ionization of intact proteins leading to poor detection sensitivity, it is important to remove or significantly minimize the introduction of these compounds into the mass analyzer. The reversed-phase, phenyl material contained in MassPREP On-Line devices successfully “traps” proteins, allowing the salts to be washed to waste prior to protein elution into the mass spectrometer.
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