Lytechinus variegatus 5SrDNA (1) of 208 bp is used for mononucleosome formation in a gel shift assay.

 Plasmid (Litmus 29) containing five copies 5SrDNA is isolated from E. coli NEB 10-beta by a standard purification procedure, digested and 208 bp DNA purified

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl 
1 mM EDTA 
pH 8.0 @ 25°C