EnGen® Lba Cas12a (Cpf1) from Lachnospiraceae bacterium ND2006 is a programmable DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) ⁽¹⁾. Targeting requires a gRNA complementary

to the target site as well as a 5’ TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the

target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~18 bases 3’ of the PAM and leaves 5’

overhanging ends. EnGen Lba Cas12a (Cpf1) has Simian virus 40 (SV40) T antigen nuclear localization

sequences (NLS) at both the N and C-termini of the protein.

EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.

Storage Temperature
-20°C

Storage Conditions
0.1 mM EDTA
50% Glycerol
500 mM NaCl
20 mM sodium acetate
0.1 mM TCEP
pH 6.0 @ 25°C

Heat Inactivation
65°C for 10 min

100 µM is equal to 15.1 mg/ml EnGen Lba Cas12a.

References

1、Zetsche, B., et. al. (2015). Cell. 163, 759-771.