EnGen® Lba Cas12a (Cpf1) from Lachnospiraceae bacterium ND2006 is a programmable DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) (1). Targeting requires a gRNA complementary
to the target site as well as a 5’ TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the
target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~18 bases 3’ of the PAM and leaves 5’
overhanging ends. EnGen Lba Cas12a (Cpf1) has Simian virus 40 (SV40) T antigen nuclear localization
sequences (NLS) at both the N and C-termini of the protein.
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
Storage Temperature
-20°C
Storage Conditions
0.1 mM EDTA
50% Glycerol
500 mM NaCl
20 mM sodium acetate
0.1 mM TCEP
pH 6.0 @ 25°C
Heat Inactivation
65°C for 10 min
100 µM is equal to 15.1 mg/ml EnGen Lba Cas12a.
References
1、Zetsche, B., et. al. (2015). Cell. 163, 759-771.
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