  Suitable for pulsed field gel electrophoresis

  Size range: 15–291 kb

  Embedded in 1% Low Melting Point (LMP) agarose

  Supplied in a GelSyringe^™ dispenser

  Suitable for 50 gel lanes

MidRange PFG Marker consists of concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1

Sam7) mixed with XhoI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe™

dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers

with intact λ DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size

range: 15-291 kb.

10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8.0 @ 25°C

-20°C

1、The photograph represents the pulsed field gel separation of Mid Range PFG Marker using a CHEF

      apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 25 seconds

      for 24 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with

      Milli-Q™ water.

2、Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is

      sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just

     above gelling temperature (~42–45°C). Allow no bubbles to form.

3、Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe

      volume scale) contains approximately 0.5µg of DNA. Each GelSyringe yields 50+ plugs.

4、Melting plugs will cause denaturation of concatemers.

5、Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel

      will cause the Lambda concatemers to denature. 

1、Ellard, J., Greci, J. and Davis, T.B. unpublished observation.