Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3′ to 5′ direction at 37°C, and can be easily heat-inactivated at 80°C for 1 minute. This enzyme has no activity on linear/circular dsDNA or RNA. Thermolabile Exonuclease I is ideal for:

• Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
• Removal of single-stranded primers for nested PCR reactions
• Removal of single-stranded DNA from dsDNA