High Sensitivity PCR
High Throughput PCR
Routine PCR
AT-rich PCR
Colony PCR
Long PCR (up to ~6 kb genomic)
OneTaq® Quick-Load® DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep Vent™
DNA Polymerases ideally suited to routine PCR applications from a variety of templates, including pure
DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA
Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient
quick-load buffer contains two commonly used tracking dyes for direct loading of PCR products on DNA
gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates
at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli
strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble
material in 30 minutes at 75°C.
5,000 units/ml
1. Barnes, W.M. (1994). Proc. Natl. Acad. Sci. USA. 91, 2216-2220.
2. Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
3. Powell, L.M. et al. (1987). Cell. 50, 831-840.
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