Product Source
An E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus 6-55 (Z. Chen)

Reagents Supplied

The following reagents are supplied with this product:

Concentration：20,000 units/ml 

Product Categories:

 

High-Fidelity (HF®) Restriction Endonucleases, 

Restriction Endonucleases: B, 

Time-Saver™ Qualified Restriction Enzymes

Applications: 

Golden Gate Assembly, 

Restriction Enzyme Digestion, 

Restriction Enzyme Digestion

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Reaction Conditions

1X CutSmart® Buffer 
Incubate at 37°C

1X CutSmart® Buffer 
50 mM Potassium Acetate 
20 mM Tris-acetate 
10 mM Magnesium Acetate 
100 μg/ml BSA 
(pH 7.9 @ 25°C)

Activity in NEBuffers

CutSmart® Buffer: 100% 
NEBuffer 1.1: 100% 
NEBuffer 2.1: 100% 
NEBuffer 3.1: 100% 

Diluent Compatibility

• Diluent B

Storage Temperature

-20°C

Storage Conditions

0.1 mM EDTA 
50% Glycerol 
1 mM DTT 
10 mM Tris-HCl 
200 μg/ml BSA 
200 mM NaCl 
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 min

Methylation Sensitivity

dam methylation: Not Sensitive 
dcm methylation: Impaired by Some Combinations of Overlapping 
CpG Methylation: Blocked by Some Combinations of Overlapping 

 Companion Products

• Gel Loading Dye, Purple (6X)
• dam-/dcm- Competent E. coli
• BsaI
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

 BsaI-HFv2 has the same specificity as BsaI (NEB #R0535), but it has been engineered for reduced star activity.