Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

• Time-Saver™ qualified for digestion in 5-15 minutes
• Isoschizomer of BsmBI
• Used in Golden Gate Assembly 
• Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
• Supplied with 1 vial of Gel Loading Dye, Purple (6X)

• Product Source

  An E. coli strain that carries the cloned Esp3I gene from Erwinia species RFL3 (A. Janulaitis).

  Reagents Supplied

  The following reagents are supplied with this product:

  	Store at (°C)	Concentration
  Gel Loading Dye, Purple (6X)	25	6 X
  CutSmart® Buffer	-20	10 X